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KMID : 1094720210260010107
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Biotechnology and Bioprocess Engineering
2021 Volume.26 No. 1 p.107 ~ p.113
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Aptamer-immobilized Gold Nanoparticles Enable Facile and On-site Detection of Staphylococcus aureus
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Lim Seong-Ha
Ryu Yeong-Chae
Hwang Byeong-Hee
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Abstract
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Staphylococcus aureus causes numerous illnesses and national loss in many countries. Therefore, to prevent food poisoning outbreaks, a rapid and straightforward on-site detection method is required without special skills or equipment. Here, we used aptamer-immobilized gold nanoparticles (aiGNPs) for the on-site colorimetric S. aureus detection. For the rapid and facile detection of S. aureus, the SA61 aptamer hybridized with thiol-modified adaptors conjugated onto the GNP surface of approximately 13 nm diameter. The synthesis of GNPs was confirmed using a transmission electron microscopy and energy dispersive spectroscopy. Images using a scanning electron microscope showed attachment of aiGNPs to S. aureus. After 30-min incubation and 20-min centrifugation, the red pellet was specifically observed with the sensitivity of OD600 0.050 equivalent to 7.5 × 106 cells/mL. Besides, aiGNPs could accurately detect S. aureus with a detection limit of OD600 0.10 equivalent to 1.5 × 107 cells/mL even in the mixed sample with Escherichia coli. Quantitative analysis was performed between relative area and cell concentration. Because this method enables fast and specific S. aureus detection without special skills or expensive equipment, aiGNPs could enable a rapid and effective on-site detection of S. aureus to prevent pathogenic outbreaks.
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KEYWORD
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aptamer, gold nanoparticle, on-site detection, Staphylococcus aureus, diagnosis
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